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Home Q & A Board Biology-Related Homework Help General Biology
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ZTian ZTian
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11 years ago
Hi,

I was doing a biology lab and it involved adding enzyme to a substrate and seeing the reaction. I was wondering why the substrate is first placed into the test tube and then the enzyme is added to it. Why isn't it the other way around? Is there a good reason for this?

Thanks!
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rainbowdropsrainbowdrops
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11 years ago
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ZTian Author
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#2
11 years ago
Quote from: rainbowdrops (11 years ago)
Actually, if you are only mixing substrate and enzyme, then it does not matter whether the substrate is added last or the enzyme is. You simply want to observe the reaction between substrate and enzyme as soon as they are mixed together (as sson as a reaction occurs) in order to avoid loss of data. So, as long as either one is added last, then it's fine.
However, if an inhibitor is involved you want to add the enzyme last because the (competitive) inhibitor binds to the active site of the enzyme, preventing substrate molecules to bind with the enzyme.

Thank you for the answer. I think it solves my question. Appreciate it.
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