× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
5
o
5
4
m
4
b
4
x
4
a
4
l
4
t
4
S
4
m
3
s
3
New Topic  
Koreanraichu Koreanraichu
wrote...
Posts: 4
Rep: 0 0
7 years ago
1X tbs w/ sample
(1X TBS buffer : 90% DW+10% 10X TBS)

<1>
sample 100ul(diluted at DW until 1000X)
DW 800ul
10X TBS 100ul

it's my co-worker's opinion. he said "sample in DW, then when you dilute in 1X TBS buffer, it's final conc. will 0.9X. negative curve occurs by conc. mismatch, and we need to 1X TBS, so this method will reduce negative curve by buffer mismatch. "

<2>
sample 100ul(1000X stock was serially diluted at 10X TBS, triple)
1X TBS 900ul(90% DW+10% 10X TBS)

it's my counter opinion. "Diluted sample is 1000X stock. then, we don't have to calculate complexly, Serial dilution in 1X TBS three times. "

I am harassing by negative curve by buffer conc. mismatch...
chip is Biotin-SAM, I using streptavidin.  Crying Face
Read 760 times

Related Topics

New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  795 People Browsing
 111 Signed Up Today
Related Images
  
 858
  
 244
  
 137
Your Opinion
Which 'study break' activity do you find most distracting?
Votes: 741