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Home Q & A Board Science-Related Homework Help Chemistry (Moderator: Laser_3)
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Jessc Jessc
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7 years ago
1. As an example of how TLC can be used to monitor the progress of a separation by column chromatography consider the following hypothetical situation. A chemist found that a mixture of 4 components (A-D) could be separated on a silica gel TLC plate using 10% diethyl ether in hexanes as the eluting solvent. The mixture was then chromatographed on a silica gel; column eluted with this same solvent mixture and 11 fractions of 15 mL each were collected. Thin-layer chromatographic analysis of the various fractions under the conditions stated above gave the results shown in the following figure. Assume the components align as A-D, with A at the top and D at the bottom of the TLC plate original mixture.

a. according to these results, which fractions should be combined to give pure samples of A, B, C, and D?

b. What fraction number still contain more than one compound? Indicate for these “mixed” fraction numbers what components of the original mixture are present?
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bio_manbio_man
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7 years ago
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fiero86,  hello-kitty,  biolife1234

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Jessc Author
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#2
7 years ago
Thanks bio_man ! You're the best
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#3
Educator
7 years ago
My pleasure. Make sure you provide feedback for all your questions because every time you do, you get 2 credits.
Jessc Author
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#4
7 years ago
Ok cool how do you do that ?
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#5
Educator
7 years ago
Feedback, i.e. simply reply back --  that's all
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