After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ...

I'm gearing more towards D, but A is also a possibility.
This piece of information should help you answer this question:
A DNA construct is an artificially constructed segment of nucleic acid that is going to be "transplanted" into a target tissue or cell. It often contains a DNA insert, which contains the gene sequence encoding a protein of interest, that has been subcloned into a vector, which contains bacterial resistance genes for growth in bacteria, and promoters for expression in the organism. A DNA construct may express wildtype protein, prevent the expression of certain genes by expressing competitors or inhibitors, or express mutant proteins, such as deletion mutations or missense mutations. A DNA construct is often used in molecular biology to analyze macromolecules such as proteins or RNA in more detail.

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I just completed the test and you will find the answers as followed. Some of my answers were wrong, but I provided the CORRECT answer. I hope this helps.....this was pretty difficult!

Make Transgenic Flies
Part 1: Prepare DNA to be incorporated into the fly genome
Quiz Answers: (Click to review)
1. Correct! Your answer: D.
2. Correct! Your answer: C.
3. Correct! Your answer: B.
4. Correct! Your answer: A.
Part 2: Prepare fly embryos
Quiz Answers: (Click to review)
1. Correct! Your answer: D.
2. Correct! Your answer: C.
Part 3: Inject fly embryos with DNA
Quiz Answers: (Click to review)
1. Correct! Your answer: C.
2. Correct! Your answer: B.
Part 4: Breed flies
Quiz Answers: (Click to review)
1. Incorrect. Your answer: C. The correct answer is D.
2. Correct! Your answer: B.
Part 5: Select transgenic progeny
Good, you found some red-eyed flies.
No quiz for this part.
Part 6: Examine light output from transgenic adults
Quiz Answers: (Click to review)
1. Correct! Your answer: C.
2. Incorrect. Your answer: B. The correct answer is A.

Use Transgenic Flies
Experiment 1: Measure per-luc gene expression while keeping lights off
Your Hypothesis: Daily light-emission oscillations will not be detected from flies kept in constant darkness.
Conclusion: Your Hypothesis is incorrect.

Quiz Answers: (Click to review)
1. Incorrect. Your answer: A. The correct answer is B.
2. Incorrect. Your answer: B. The correct answer is A.
3. Incorrect. Your answer: A. The correct answer is B.
Experiment 2: Measure per-luc gene expression while changing light on-off times
Your Hypothesis: Changing the light-dark cycles that flies are kept in resets the flies' internal clock, resulting in a shift of the peaks and troughs in the daily light-emission oscillations.
Conclusion: Your Hypothesis is correct!

Quiz Answers: (Click to review)
1. Correct! Your answer: B.
2. Correct! Your answer: A.
3. Incorrect. Your answer: B. The correct answer is A.
Experiment 3: Examine different fly body parts for per-luc expression
Your Hypothesis: Oscillating per gene expression (measured as light emissions) is found only in the Drosophila's wings.
Conclusion: Your Hypothesis is incorrect.

Quiz Answers: (Click to review)
1. Correct! Your answer: D.
2. Incorrect. Your answer: B. The correct answer is D.