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Home Q & A Board Biology-Related Homework Help Cell Biology
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allan2009 allan2009
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13 years ago
Primers need to be in excess to the template DNA. Calculate the molar concentration ratio of template to primer?

DNA template    (50ng/ul)         1ul
Primer S361(+)  (125ng/ul)        1ul
Primer S361(-)   (125ng/ul)        1ul

dNTPs 6ul, 10Xbuffer 5ul, DNA polymerase 1ul.

filled with H2O (final volume 50ul)

The template DNA has been diluted 1:4 from a stock solution of 200 ng/ul; and the primers have been diluted 1:8 from stock solution of 1000 ng/ul.
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#1
Educator
13 years ago
Never done this problem before, but I did a search for you and perhaps these sources could help...

Effects of Primer Concentration

Sample Calculations

Quantitative Analysis of the PCR Reaction

Reply back if they were actually helpful.
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