|
Title: Microbial genetics problem solving Post by: Gordon on Apr 20, 2014 These problems aren't making sense, please help.
You do a conjugation experiment in which you mate Hfr leu- , strr , ampr , purr, and F- leu+, strs, amps, purs. In all cases resistance is dominant. Strr is closest to the fertility factor. When plated on minimal media containing the supplements listed below you obtain the following data: strr ampr purr 500 colonies strr ampr purs 0 colonies strr amps purr 200 colonies strr amps purs 10 colonies If a map unit is equal to 1% recombination then what are the map distances? You are mapping the bacterial genome of a strain of E. coli. You set up the following cross. Hfr ampr, his+, pro+, purs, thr+, trp- and F- amps, his-, pro-, purr, thr-, trp+. Thr is closes to the fertility factor. You plate these cells on minimal media with supplements. After one hour you interrupt the mating and obtain colonies with the following genotypes. amps, his+, pro+, purs, thr-, trp- 647 colonies ampr, his+, pro+, purs, thr-, trp- 102 colonies amps, his-, pro+, purr, thr-, trp+ 1000 colonies ampr, his+, pro+, purs, thr+, trp- 0 colonies amps, his+, pro+, purr, thr-,trp+ 875 colonies amps, his+, pro+, purr, thr-, trp- 802 colonies What is the gene order and how did you determine this order? Title: Re: Microbial genetics problem solving Post by: yesayi91 on Apr 26, 2014 I am interested in this question as well
Title: Re: Microbial genetics problem solving Post by: bio_man on Apr 26, 2014 Hey Gordon, could you give us an update here, if possible?
Title: Re: Microbial genetics problem solving Post by: Gordon on Apr 26, 2014 Hey Gordon, could you give us an update here, if possible? Hey bio_man yep, this is just your basic microbial genetics i figured it out! Basically for the first one you look at your 0 colony offspring to determine gene order and you turn your recipient ( F- ) and your donor ( Hfr ) into your offspring. Once you get them figured out you put those two over the total and times by 100. For the second one you look at the Hfr ampr his+ pro+ purs thr+ and trp- and see if they changed, like for the 1000 colonies you can see that pro+ changed because the others are all opposite and pro+ is still pro+ therefore it changed and you do that for all of them but you ignore pro+ because you already did it and you ignore the ones you already used until you get down to the last one and you have your order.Title: Re: Microbial genetics problem solving Post by: bio_man on Apr 26, 2014 I am glad you figured it out, awesome... Also, thank you for the update.
|