Definition for Difference between revisions of "Probe"

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(Created page with "A fragment of DNA or RNA which is labeled in some way (often incorporating 32P or 35S), and which is used to hybridize with the nucleic acid in which you are interested. For exam...")
 
 
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A fragment of DNA or RNA which is labeled in some way (often incorporating 32P or 35S), and which is used to hybridize with the nucleic acid in which you are interested. For example, if you want to quantitate the levels of alpha subunit mRNA in a preparation of pituitary RNA, you might make a radiolabeled RNA in-vitro which is complementary to the mRNA, and then use it to probe a Northern blot of the pit RNA. A probe can be radiolabeled, or tagged with another functional group such as biotin. A probe can be cloned DNA, or might be a synthetic DNA strand. As an example of the latter, perhaps you have isolated a protein for which you wish to obtain a cDNA or genomic clone. You might (pay to) microsequence a portion of the protein, deduce the nucleic acid sequence, (pay to) synthesize an oligonucleotide carrying that sequence, radiolabel it and use it as a probe to screen a cDNA library or genomic library. A better way is to call up someone who already has the clone.
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In [[biology]], a '''probe''' is a fragment of DNA or RNA which is labeled in some way (often incorporating 32P or 35S), and which is used to hybridize with the nucleic acid in which you are interested. For example, if you want to quantitate the levels of alpha subunit mRNA in a preparation of pituitary RNA, you might make a radiolabeled RNA in-vitro which is complementary to the mRNA, and then use it to probe a Northern blot of the pit RNA.  
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A probe can be radiolabeled, or tagged with another functional group such as biotin. A probe can be cloned DNA, or might be a synthetic DNA strand. As an example of the latter, perhaps you have isolated a protein for which you wish to obtain a cDNA or genomic clone.

Latest revision as of 15:16, 28 February 2018

In biology, a probe is a fragment of DNA or RNA which is labeled in some way (often incorporating 32P or 35S), and which is used to hybridize with the nucleic acid in which you are interested. For example, if you want to quantitate the levels of alpha subunit mRNA in a preparation of pituitary RNA, you might make a radiolabeled RNA in-vitro which is complementary to the mRNA, and then use it to probe a Northern blot of the pit RNA.

A probe can be radiolabeled, or tagged with another functional group such as biotin. A probe can be cloned DNA, or might be a synthetic DNA strand. As an example of the latter, perhaps you have isolated a protein for which you wish to obtain a cDNA or genomic clone.

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