A 0.51 m aqueous solution of an unknown solute has a boiling point elevation of 0.62°C. The boiling point elevation of a 0.51m solution of a nonionizing molecular solute in water is 0.26°C. How many moles of particles are formed per mole of solute when the unknown solute is dissolved in water?
The table above discusses freeze drying, but I don't think the OP needs that kind of information.
For short-term storage:
Working bacterial stocks can be streaked onto agar plates and stored at 4°C for daily or weekly use. Culture dishes should be wrapped with laboratory sealing film (plastic or paraffin) and stored upside down (agar side up) to minimize contamination and to keep both the culture and agar properly hydrated. Some bacterial strains can be stored for up to 1 year at 4°C in agar stab cultures.
For long-term storage:
The temperature at which frozen bacteria are stored affects how long they can be stored while remaining viable. Freezing and thawing cells at an appropriate rate and maintaining the frozen stocks at the proper storage temperature help to minimize damage from the freezing process. Also, the greater the cell density, the better the recovery is after thawing the cells. For most bacteria, a density of 107 cells/mL will result in adequate recovery if all conditions are properly maintained.
Question 1: Is it possible to take 100ml of medium with already fully developed bacterial culture inside and mix it with 900ml of pure medium to effectively grow another 1000ml of bacterial culture?
Yes, it will grow in theory if there's an energy source found within the medium.
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This process would allow me to keep "cloning" the initial bacterial colony indefinitely without the need of purchasing freeze-dried samples every time I need to run experiments.
Yes, good strategy.
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Question 2: Is it possible to keep the already fully developed bacterial culture in a 100ml medium for longer periods of time (months)?
The specific length of time that a culture will remain viable in a given storage condition is dependent upon the bacterial strain. According to this article:
Being isolated and differentiated, Gluconobacter can be maintained on various liquid (beer) and solid media. Table 1 gives a survey on recommended solid media. Agar cultures should be kept at 4 °C and transferred monthly. Strains can be kept frozen at –75 °C in the presence of 24% (v/v) glycerol or dimethyl sulfoxide (10%, v/v). Freeze-dried strains will remain alive for several years.
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