Definition for Band shift assay
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Revision as of 14:03, 27 July 2012 by Howard (Talk | contribs) (Created page with "Also called gel shift or electrophoretic mobility shift is a method for detecting DNA-binding proteins. A band shift is observed when a protein forms a complex with a DNA fragmen...")
Also called gel shift or electrophoretic mobility shift is a method for detecting DNA-binding proteins. A band shift is observed when a protein forms a complex with a DNA fragment, because complexes of protein and DNA migrate through a non-denaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides.
