Definition for Expression clone
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Revision as of 03:37, 26 December 2014 by Duddy (Talk | contribs) (Created page with "This is a clone (plasmid in a bacteria, or maybe a l phage in bacteria) which is designed to produce a protein from the DNA insert. Mammalian genes do not function in bacteria, s...")
This is a clone (plasmid in a bacteria, or maybe a l phage in bacteria) which is designed to produce a protein from the DNA insert. Mammalian genes do not function in bacteria, so to get bacterial expression from your mammalian cDNA, you would place its coding region (i.e. no introns) immediately adjacent to bacterial transcription/translation control sequences. That artificial construct (the "expression clone") will produce a pseudo-mammalian protein if put back into bacteria. Often, that protein can be recognized by antibodies raised against the authentic mammalian protein, and vice versa.