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Kmoy Kmoy
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9 years ago
Medium 1099 contains coy's solution, 10% fetal bovine serum, 20mM HEPES, 10 microgram/ml insulin, 1x beta-mercapatoethanol, and 2mM glutamine.

You have the following reagents: coy's solution containing 7mM HEPES as well as stocks of 1M HEPES solution, 4mg/ml insulin, 1000x beta-mercapatoethanol, and 200 mM glutamine. How would you make the medium1099? Show and explain calculations
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