Definition for Indirect ELISA
From Biology Forums Dictionary
An indirect ELISA test is used to detect the presence of antibodies. A known antigen is fixed to a small well, and the patient’s serum is added. Patient’s antibodies will react with the antigen in the well. Antihuman immunoglobulins bound to an enzyme are added to the well. The antihuman immunoglobulins will bind to the patient’s antibodies. Substrate for the enzyme is then added and a positive reaction indicating presence of the antibody in the patient’s serum is shown by the enzyme–substrate reaction.
Also see: direct ELISA.