Which of the following is not a feature of miRNAs?
A. Primary miRNA transcripts have internal complementary sequences capable of forming hairpin structures.
B. Precursor miRNAs have internal complementary sequences capable of forming hairpin structures.
C. miRNAs are exported to nuclease in their single-stranded form.
D. Precursor miRNAs are approximately 6070 nucleotides long.
Which of the following characteristics of DNA-dependent DNA synthesis is not the same for
DNA-dependent RNA synthesis?
A. Synthesis requires a template.
B. Initiation involves the recognition of a specific DNA sequence.
C. Initiation of synthesis requires a primer.
D. Synthesis is catalyzed in the 5' to 3' direction.
E. All of the choices given are the same for both DNA and RNA synthesis.
Which repair mechanism requires light energy?
A. nucleotide excision repair (NER)
B. base excision repair (BER)
C. mismatch repair (MMR)
D. photoreactivation
E. transcription coupled repair
Supercoiling of double-stranded DNA is called its ______________ structure.
A. primary
B. secondary
C. tertiary
D. quaternary
E. duplex
You wish to determine the cellular location of a protein but unfortunately it is not particularly antigenic (i.e., it can't develop a strong antibody response). How could you solve this problem?
A. Create a fusion protein with an epitope tag that can be visualized by incubation with fluorescently tagged antibody to the epitope.
B. Create a fusion protein with an epitope tag that is fluorescent without the addition of antibodies.
C. Create a fusion with green fluorescent protein (GFP). The fusion protein will fluoresce when streptavidin is added.
D. Express the protein as a fusion with biotin. The addition of GFP will cause fluorescence.
E. None of these methods will work.
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