Some proteins overexpressed at high levels resulting in the formation of inclusion bodies can abolish the goal of producing large quantities of active protein. What could be done to minimize this issue?
A) Codon optimize the gene.
B) Decrease the number of biobricks in the vector.
C) Simultaneously produce intracellular chaperonins.
D) Switch to an expression host with a larger intracellular volume.
Polyvalent vaccines using vaccinia virus are highly favored by doctors and physicians but are especially challenging for those who develop them, because
A) coat proteins form a relatively rigid structure and do not allow much space for additional proteins to be expressed.
B) multiple foreign proteins simultaneously synthesized often disrupts each other's activity.
C) vaccinia and most other viruses engineered for vaccines contain only one restriction site for cloning in their genome.
D) virus genetic manipulation uses transfection, which is an inherently inefficient process.
Recognizing pathogens that contain multiple unique proteins which enable the human immune system to recognize just one and mount an effective response has opened the door on development of some vaccines only being
A) attenuated carrier viruses.
B) monovalent.
C) subunit vaccines.
D) purified protein administered.
Which construct would be MOST useful in studying translational control?
A) gene fusion
B) operon fusion
C) protein fusion
D) shuttle vector
If a protein to be overexpressed is toxic to the expression host, it is best to select an expression vector that
A) is compatible with a binary vector able to be regulated.
B) is inducible.
C) has a relatively low copy number per cell.
D) prevents folding of the overexpressed protein into its toxic form.
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