Hey Im just working on my lab for Physio Ex 8.0 and Im not sure if this is right? Maybe somone has done these questions and knows if what I have said is correct.
1.1. At a given glucose concentration (mM), how does the amount of time it takes to reach equilibrium change with the number of carrier proteins used to build the membrane? (1 mark)
-The time is faster to reach the equilibriums because increasing the number of carriers increased the rate of diffusion. In other words, as the glucose carrier proteins increase , the amount of time it takes to reach equilibrium decreases.
1.2. Is there a change in diffusion rate of Na+ and Cl- with the number of receptors? What is the mechanism of the Na+ and Cl- transport through the membrane? (1 mark)
-No, there is no change in diffusion rate of Na+ and Cl- with the number of receptors.
- The mechanism of the Na and Cl transport through the membrane is by simple diffusion. This occurs because the glucose is being actively transported across not the NaCl (salt). If it did the amount of NaCl transported would increase with the number of receptors. However, the receptors are specific for glucose in this case.
Osmosis
2.1. Describe the relationship of solute concentration to solvent concentration in osmosis. (1 mark)
- Explain the relationship between solute concentration and osmotic pressure? Osmotic Pressure is the amount of pressure that needs to be supplied to the 2nd beaker in order to prevent water entry from the 1st beaker. Solute concentration depends on osmosis.
2.2. Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCL on the other side? Is so, which solution was generating the pressure? (1 mark)
- Yes, there would be pressure generated if you had 9mM glucose on one side of a 200MWCO membrane and 9mM NaCL on the other side. This is because the solutes on either side of the membrane are different even though there concentrations in mM are the same.
- The side with the glucose would be generating the pressure.
Active Transport
3.1. If you placed 9 mM NaCl on one side of a membrane and a 15 mM NaCl on the other side, would there be movement of the NaCl? If so, in what direction? Explain why? (1 mark)
- Yes, there would be movement of the NaCl because they have different concentrations on eather side of the membrane. When they are allowed to penetrate the semipermeable membrane the side with 15mM of NaCl will move towards the side with 9mM NaCl until both sides are balanced. When I attempted this both sides found equilibrium of 12mM NaCl on both sides.
3.2. By what mechanism does the active transport of sodium lead to osmotic flow of water across a membrane? (1 mark)
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