Which may result in Gram-positive bacteria appearing to be Gram-negative?

Which may result in Gram-positive bacteria appearing to be Gram-negative?
 
  A.
  Decolorizing too long
 
  B.
  Decolorizing too short
 
  C.
  Using old cultures
 
  D.
  Using young cultures
 
  E.
  Decolorizing too long AND using old cultures

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A wound created by the drag of a knife across skin can be classified as
 
  A. puncture.
  B. incised.
  C. lacerated.
  D. contused.

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The order of reagents in the Gram stain reaction are
 
  A. safranin, alcohol, methylene blue, iodine.
  B. crystal violet, iodine, alcohol, safranin.
  C. methylene blue, alcohol, safranin.
  D. crystal violet, alcohol, iodine, safranin.

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The Gram stain and the endospore stain both use
 
  A.
  crystal violet.
 
  B.
  iodine.
 
  C. safranin.
  D.
  malachite green.
 
  E.
  acidic dyes.

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The normal habitat of Clostridium tetani is
 
  A. humans.
  B. animals.
  C. plants.
  D. soil and dust.