In principle, a type II restriction endonuclease with an 8-nucleotide recognition sequence should ...

In principle, a type II restriction endonuclease with an 8-nucleotide recognition sequence should cut 1 in every 48 nucleotide positions.
 
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Modification enzymes typically methylate specific bases within the recognition sequence to prevent digestion of the nucleotide sequence by restriction endonucleases.
 
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One fundamental technique of genetic engineering includes the ability to cut DNA into random fragments.
 
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One problem with both BACs and YACs is that genetic regions of these chromosomes cannot be subcloned.
 
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Although various codons often code for the same amino acid, it is important to choose the codon preferred by the expression host itself.
 
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