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Home Q & A Board Biology-Related Homework Help General Biology
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Andrearcarter95 Andrearcarter95
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6 years ago
In Southern blotting DNA fragments are separated by _____, transferred to _____, and visualized by _____.
  1.size / DNA-binding membrane / ethidium bromide
  2.charge / filters / colored dye
  3.heating / agarose gel / fluorescence under UV light
  4.size / DNA-binding membrane / hybridizing with radioactively labeled probes

Question 2

In the polymerase chain reaction (PCR) the DNA is first heated to break the hydrogen bonds between the two polynucleotide strands, producing two single-stranded molecules that will serve as templates. Which of the following choices would be the second step in PCR?
  1.The mixture is cooled and primers bind to complementary DNA regions.
  2.Taq polymerase synthesizes a complementary DNA strand for each single-stranded molecule.
  3.The mixture is heated and primers bind to complementary DNA regions.
  4.The DNA is cut into fragments with a restriction enzyme.
  5.The DNA is stained.
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elieli
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6 years ago
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6 years ago
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