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Home Q & A Board Biology-Related Homework Help Medicine Nursing and Clinical
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colleen colleen
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12 years ago
A throat swab is taken from a patient with a respiratory infection. After 24 hours, 1- to 3-mm colonies develop, which are round, mucoid, nonpigmented, and dimpled in the middle. What is the most likely pathogen, and how could you confirm the diagnosis on the culture?
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Sunshine ☀ ☼

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12 years ago
The colonies described are characteristic of Streptococcus pneumoniae. Diagnosis of this pathogen is confirmed if they are alpha-hemolytic on blood agar when grown aerobically and beta-hemolytic when grown anaerobically. Pneumococcal colonies can be differentiated from other colonies of alpha-hemolytic strains by adding a drop of bile to a colony. The bile triggers chemicals in S. pneumoniae that lyse the cells. The colony dissolves in a few minutes, confirming that they are pneumococcal colonies.
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