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oark oark
wrote...
Posts: 39
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11 years ago
I do have a specific test to run on the extract to see the enzyme activity.
Will sonication work? at what dB level?
Ideas?
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wrote...
11 years ago
Sonication protocols for E coli protein recovery
http://aberdeen.ac.uk/~mmb023/protocol/bactprep.htm
http://wolfson.huji.ac.il/expression/procedures/cell_lysis/Lysis_of_bacterial_cells.html
http://www.protocol-online.org/biology-forums/posts/24496.html
http://webcache.googleusercontent.com/search?q=cache%3AXA29iamNm9EJ%3Awww.matrical.com%2FLiterature%2FBacteria%2520Lysis%2520App%2520Note%2520_Edited_1.pdf+bacteria+sonication+protocol&hl=en&gl=us
http://cshprotocols.cshlp.org/cgi/content/abstract/2006/23/pdb.prot4532
http://www.springerlink.com/content/p041h74u8394204j/
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