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Home Q & A Board Biology-Related Homework Help Cell Biology
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smokebomb13 smokebomb13
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Posts: 125
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11 years ago
?  7. Which of the following primers can amplify the following DNA segment by PCR?
?     GACTGGTCGCGGATAGCGTATAATATTTC GGAATACGGTATACCCGGATACGGATA
?a) 5'CTGACCAGCGCC and 5'GACTGGTCGCGG
?b) 5'ATAGGCATAGGC and 5'CCGCGACCAGTC
?c) 5'ATAGGCATAGGC and 5'TATCCGTATCCG
?d) 5'ATAGGCATAGGC and 3'CTGACCAGCGCC
?e) 5'TATCCGTATCCG and 3'GGCGCTGGTCAG

I know the answer is e. But I don't understand how to approach this type of question.

Thanks in advance.
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wrote...
#1
11 years ago
The two primers need to be complementary to the 3' (three prime) ends of each of the sense and anti-sense strand of the DNA target.

So 3'GGCGCTGGTCAG is complementary to the 3' end of the sense and 5'TACCGTATTCCG is complementary to the 3' of the anti-sense.

uhh!! and the e. statement should be corrected to 5'TACCGTATTCCG and 3'GGCGCTGGTCAG a T was misplaced...
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