(Solved) PhysioEx 8.0

lcv123

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13 years ago

PhysioEx 8.0 Exercise 1 ISBN 0-32154900-7 ISBN 978-0-321-54900-6

I NEED HELP WITH EXERCISE 1 ON CELL TRANPORT MECHANISMS AND PERMEABILITY I HAVE NONE OF IT ASNWEARED

THANK YOU

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duddy

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Staff Member

13 years ago

Sure, I could assist you with that, but could you tell me exactly which question (like actually state which activity and/or questions)?

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lcv123 Author

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13 years ago

1.   What is the molecular weight of Na+? 22.99
2.   What is the molecular weight of Cl-? 35.45
3.   Which MWCO dialysis membranes allowed both of these ions through?
4.   Which materials diffused from the left beaker to the right beaker? All solutes except albumin are able to diffuse into the right beaker
5.   Which did not? Why? Albumin, because it is too large to diffuse

Activity 2: Simulating Dialysis

1.   What happens to the urea concentration in the left beaker (the patient)?
2.   Why does this occur?

Facilitated Diffusion
Activity 3: Facilitated Diffusion

1.   At a given glucose concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membranes?
2.   Does the diffusion rate of Na+/Cl- change with the number of receptors?
3.   What is the mechanism of the Na+/Cl- transport?
4.   If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion?
5.   Does being unable to observe diffusion necessarily mean that diffusion is not taking place?

Osmosis
Activity 4: Osmosis

1.   Did you observe any pressure changes during the experiment? If so, in which beaker(s), and with which membranes? Using the 20 MWCO membrane results in an osmotic pressure increase using any of the solutes
2.   Why?
3.   Did the Na+/Cl- diffuse from the left beaker to the right beaker? If so, with which membrane(s)?
4.   Why?
5.   Explain the relationship between solute concentration and osmotic pressure?
6.   Does diffusion allow osmotic pressure to be generated?
7.   Would pressure be generated if solute concentrations were equal on both sides of the membrane?
8.   Why or why not?
9.   Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure?
10.   Would pressure be generated if you had 9 mM albumin on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure?

Filtration
Activity 5: Filtration

1.   What were the results of your initial membrane analysis?
2.   Does the membrane MWCO affect filtration rate?
3.   Does the amount of pressure applied affect the filtration rate?
4.   Did all solutes pass through all the membranes?
5.   If not, which one(s) did not?
6.   Why?
7.   How can the body selectively increase the filtration rate of a given organ or organ system?

Active Transport
Activity 6: Active Transport

1.   At the end of this experimental run, did the Na+/Cl- move from the left vessel to the right vessel?
2.   Why?
3.   As the run progresses, the concentrations of the solutes will change in the windows next to the two beakers. The rate will slow down markedly, then stop before completed. Why?
4.   Does the amount of NaCl/KCl transported change?
5.   Does the amount of solute transported across the membrane change with an increase in carriers or pumps?
6.   Is one solute more affected than the other?
7.   Does the membrane you “built” allow simple diffusion?
8.   If you placed 9 mM NaCl on one side of the membrane and 15 mM on the other side, would there be movement of the NaCl? Why?
9.   Does the amount of ATP added make any difference?

so i

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lcv123 Author

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13 years ago

the first ones that have answers i have tried to do but im not even sure if those are correct? i am really stressing out about this class! Thank you very much for your time hope you can help me! Either way thanks a lot!!!

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duddy

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lcv123 Author

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13 years ago

THANX hear is what i came up with what you gave me im posting it hear so maybe it can help some one

1.   What is the molecular weight of Na+? 22.99
2.   What is the molecular weight of Cl-? 35.45
3.   Which MWCO dialysis membranes allowed both of these ions through?50, 100 and 200
4.   Which materials diffused from the left beaker to the right beaker Urea, NaCl and glucose diffused
5.   Which did not? Why? Albumin, because it is too large to diffuse

Activity 2: Simulating Dialysis

1.   What happens to the urea concentration in the left beaker (the patient)? Its concentration gradient changes and causes it to move down
2.   Why does this occur? It happened because the more concentrated it became it causes the molecules to move down.

Facilitated Diffusion
Activity 3: Facilitated Diffusion

1.   At a given glucose concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membranes? The time is faster to rach the equilibriums.
2.   Does the diffusion rate of Na+/Cl- change with the number of receptors? YES
3.   What is the mechanism of the Na+/Cl- transport?
4.   If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion? NO there is no diffusion taking place
5.   Does being unable to observe diffusion necessarily mean that diffusion is not taking place? yes you should be able to tell when equilibrium is reached

Osmosis
Activity 4: Osmosis

1.   Did you observe any pressure changes during the experiment? If so, in which beaker(s), and with which membranes? Yes in the left beaker at the membrane 20
2.   Why?
3.   Did the Na+/Cl- diffuse from the left beaker to the right beaker? If so, with which membrane(s)? No with any of the membranes.
4.   Why? Because there is equal pressure on both sides
5.   Explain the relationship between solute concentration and osmotic pressure? as solute goes higher do does the osmotic pressure.
6.   Does diffusion allow osmotic pressure to be generated? Yes
7.   Would pressure be generated if solute concentrations were equal on both sides of the membrane? no it does not generate
8.   Why or why not?
9.   Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure? yes in the side with the glucose.
10.   Would pressure be generated if you had 9 mM albumin on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure? Yes, The Albumin was generating the pressure .

Filtration
Activity 5: Filtration

1.   What were the results of your initial membrane analysis?
2.   Does the membrane MWCO affect filtration rate? Yes, the smaller the mwco the smaller the pore sizes are and they affect the filtration rate.
3.   Does the amount of pressure applied affect the filtration rate? yes.
4.   Did all solutes pass through all the membranes? no.
5.   If not, which one(s) did not? urea was the only one not present.
6.   Why? Urea does not diffuse freely.
7.   How can the body selectively increase the filtration rate of a given organ or organ system? By increasing or decreasing the blood supply to the given organ or system.

Active Transport
Activity 6: Active Transport

1.   At the end of this experimental run, did the Na+/Cl- move from the left vessel to the right vessel? No it did not
2.   Why? Because its supposed to move form a lower to a higher concentration area in this case it was the opposite.
3.   As the run progresses, the concentrations of the solutes will change in the windows next to the two beakers. The rate will slow down markedly, then stop before completed. Why? Because there is a lack of atp.
4.   Does the amount of NaCl/KCl transported change? No it did not change
5.   Does the amount of solute transported across the membrane change with an increase in carriers or pumps? No it did not
6.   Is one solute more affected than the other? No they were both equal.
7.   Does the membrane you “built” allow simple diffusion? no it does not
8.   If you placed 9 mM NaCl on one side of the membrane and 15 mM on the other side, would there be movement of the NaCl? Why? There was no movement when I conducted the experiment because it wasthe same chemical.
9.   Does the amount of ATP added make any difference? Yes

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hebam1994

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duddy

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Staff Member

13 years ago

nice, thanks lcv123

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kkarg78

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13 years ago

Your Questions: Activity 2: Simulating Dialysis

1. What happens to the urea concentration in the left beaker (the patient)?
2. Why does this occur?

Facilitated Diffusion
Activity 3: Facilitated Diffusion

1. At a given glucose concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membranes?
2. Does the diffusion rate of Na+/Cl- change with the number of receptors?
3. What is the mechanism of the Na+/Cl- transport?
4. If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion?
5. Does being unable to observe diffusion necessarily mean that diffusion is not taking place?

Answers:
Activity 2: Simulating Dialysis

1. What happens to the urea concentration in the left beaker (the patient)? Urea moved from the left to the right through the 200 MWCO membrane.
2. Why does this occur? It happened because the more concentrated it became it causes the molecules to move down.

Facilitated Diffusion
Activity 3: Facilitated Diffusion

1. At a given glucose concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membranes? Increasing the number of carriers increases the rate of diffusion.
2. Does the diffusion rate of Na+/Cl- change with the number of receptors? No
3. What is the mechanism of the Na+/Cl- transport? Simple diffusion
4. If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion? No, because they are already balance. Another words they have reached equilibrium.
5. Does being unable to observe diffusion necessarily mean that diffusion is not taking place? No

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hebam1994

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kkarg78

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12 years ago

I really could use the help with this lab.....I really am not having any luck with it?

PhysioEx 8.0 Exercise 1

1. What is the molecular weight of Na+? 22.99
2. What is the molecular weight of Cl-? 35.45
3. Which MWCO dialysis membranes allowed both of these ions through?
4. Which materials diffused from the left beaker to the right beaker? All solutes except albumin are able to diffuse into the right beaker
5. Which did not? Why? Albumin, because it is too large to diffuse

Activity 2: Simulating Dialysis

1. What happens to the urea concentration in the left beaker (the patient)?
2. Why does this occur?

Facilitated Diffusion
Activity 3: Facilitated Diffusion

1. At a given glucose concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membranes?
2. Does the diffusion rate of Na+/Cl- change with the number of receptors?
3. What is the mechanism of the Na+/Cl- transport?
4. If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion?
5. Does being unable to observe diffusion necessarily mean that diffusion is not taking place?

Osmosis
Activity 4: Osmosis

1. Did you observe any pressure changes during the experiment? If so, in which beaker(s), and with which membranes? Using the 20 MWCO membrane results in an osmotic pressure increase using any of the solutes
2. Why?
3. Did the Na+/Cl- diffuse from the left beaker to the right beaker? If so, with which membrane(s)?
4. Why?
5. Explain the relationship between solute concentration and osmotic pressure?
6. Does diffusion allow osmotic pressure to be generated?
7. Would pressure be generated if solute concentrations were equal on both sides of the membrane?
8. Why or why not?
9. Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure?
10. Would pressure be generated if you had 9 mM albumin on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure?

Filtration
Activity 5: Filtration

1. What were the results of your initial membrane analysis?
2. Does the membrane MWCO affect filtration rate?
3. Does the amount of pressure applied affect the filtration rate?
4. Did all solutes pass through all the membranes?
5. If not, which one(s) did not?
6. Why?
7. How can the body selectively increase the filtration rate of a given organ or organ system?

Active Transport
Activity 6: Active Transport

1. At the end of this experimental run, did the Na+/Cl- move from the left vessel to the right vessel?
2. Why?
3. As the run progresses, the concentrations of the solutes will change in the windows next to the two beakers. The rate will slow down markedly, then stop before completed. Why?
4. Does the amount of NaCl/KCl transported change?
5. Does the amount of solute transported across the membrane change with an increase in carriers or pumps?
6. Is one solute more affected than the other?
7. Does the membrane you “built” allow simple diffusion?
8. If you placed 9 mM NaCl on one side of the membrane and 15 mM on the other side, would there be movement of the NaCl? Why?
9. Does the amount of ATP added make any difference?

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dustinjh

wrote...

12 years ago

Activity 1: Simulating Simple diffusion

1.   What is the molecular weight of Na+? 22.99
2.   What is the molecular weight of Cl-? 35.45
3.   Which MWCO dialysis membranes allowed both of these ions through? 50, 100, and 200 MWCO Membranes.
4.   Which materials diffused from the left beaker to the right beaker? NA+/CL-, Urea, and glucose diffused.
5.   Which did not? Why? The albumin did not diffuse because the molecular weight of the albumin exceeded the molecular weight cut off of the highest membrane.

Activity 2: Simulating Dialysis

1.   What happens to the urea concentration in the left beaker (the patient)? The urea is lowered to half of the original level in the patient.
2.   Why does this occur? The solutions on either side of the membrane are attempting to equalize the amount of urea, so half of the urea passes from the left beaker to the right beaker.

Facilitated Diffusion
Activity 3: Facilitated Diffusion

1.   At a given glucose concentration, how does the amount of time it takes to reach equilibrium change with the number of carriers used to “build” the membranes? As the carriers are increased in the membrane, the time necessary to equalize decreases.
2.   Does the diffusion rate of Na+/Cl- change with the number of receptors? No it does not change.
3.   What is the mechanism of the Na+/Cl- transport? Simple Diffusion.
4.   If you put the same amount of glucose in the right beaker as in the left, would you be able to observe any diffusion? No, because the two solutions are already equal.
5.   Does being unable to observe diffusion necessarily mean that diffusion is not taking place? No, because the rate of diffusion might be equal across the membrane, thus causing no net gain or loss in the solutions.

Osmosis
Activity 4: Osmosis

1.   Did you observe any pressure changes during the experiment? If so, in which beaker(s), and with which membranes? The NA+/CL- solution was at 306 pressure in the left beaker with the 20 MWCO membrane and 0 left and 0 right pressure for the other three membranes. The glucose was at 153 pressure left with the 20, 50 and 100 MWCO membranes and 0 left and 0 right pressure with the 200 membrane. The Albumin was at 153 pressure left with all four membranes.
2.   Why?
The NA+/CL- had the highest pressure due to the fact that is has twice the ions formed. For the rest of the changes, water flows from the lower solute concentration to the highest solute concentration, thus causing an pressure increase when the solutes are unable to diffuse across the membrane.
3.   Did the Na+/Cl- diffuse from the left beaker to the right beaker? If so, with which membrane(s)? Only in the 50, 100, and 200 MWCO membranes.
4.   Why? The molecular weight of the NA+/CL- was small enough to allow diffusion across the 50, 100, and 200 MWCO membranes.
5.   Explain the relationship between solute concentration and osmotic pressure? Water flows from the area with lowest solute concentration to the area with higher solute concentration, thus increasing the pressure in the area with higher solute concentration.
6.   Does diffusion allow osmotic pressure to be generated? No because the solutes are allowed to diffuse across the membrane, thus equalizing the solute concentration.
7.   Would pressure be generated if solute concentrations were equal on both sides of the membrane? No
8.   Why or why not? There would be no water flow across the membrane.
9.   Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure? No, because the membrane allows diffusion of both the solutes in this case.
10.   Would pressure be generated if you had 9 mM albumin on one side of a 200 MWCO membrane and 9 mM NaCl on the other side? If so, which solution was generating the pressure? Yes, pressure increases on the side containing the albumin due to the fact that the albumin cannot diffuse across the membrane.

Filtration
Activity 5: Filtration

1.   What were the results of your initial membrane analysis? All solutes were present.
2.   Does the membrane MWCO affect filtration rate? As the MWCO increases, the rate of filtration increases.
3.   Does the amount of pressure applied affect the filtration rate? As the pressure increases, the rate of filtration also increases.
4.   Did all solutes pass through all the membranes? No.
5.   If not, which one(s) did not? Nothing passed through the 20 MWCO Membrane. NA+/CL- passed through the 50, 100, and 200 MWCO membranes. Urea passed through the 100 and 200 MWCO membranes. Glucose passed through the 200 MWCO membrane. Charcoal did not pass through any membranes.
6.   Why? The Molecular weight of the solute determines whether or not the solute will be able to pass through a certain membrane with a certain MWCO.
7.   How can the body selectively increase the filtration rate of a given organ or organ system? The body can increase the blood flow to certain organs or organ systems to increase the filtration rate, thus increasing the pressure.

Active Transport
Activity 6: Active Transport

1.   At the end of this experimental run, did the Na+/Cl- move from the left vessel to the right vessel? No.
2.   Why? Active transport requires movement from both higher to lower concentration as well as movement from lower to higher concentration.
3.   As the run progresses, the concentrations of the solutes will change in the windows next to the two beakers. The rate will slow down markedly, then stop before completed. Why? The amount of available ATP is exhausted, thus there is no fuel for the active transport.
4.   Does the amount of NaCl/KCl transported change? Yes.
5.   Does the amount of solute transported across the membrane change with an increase in carriers or pumps? Yes.
6.   Is one solute more affected than the other? The NA+/CL- solute.
7.   Does the membrane you “built” allow simple diffusion? No.
8.   If you placed 9 mM NaCl on one side of the membrane and 15 mM on the other side, would there be movement of the NaCl? Why? No because this would rely solely on simple diffusion and not active transport.
9.   Does the amount of ATP added make any difference? No.

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vulture

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bio_man

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#10

Educator

12 years ago

Thank for uploading...

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alexa_daneluk

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#11

12 years ago

The ones I dont get because they have been answered different in every thred are:

1. 2.2. Would pressure be generated if you had 9 mM glucose on one side of a 200 MWCO membrane and 9 mM NaCL on the other side? Is so, which solution was generating the pressure? (1 mark)

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how_mendel

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#12

12 years ago

Glucose

larger molecule.

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PhysioEx 8.0 - Exercise 1

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