1.Compare the genomic DNA sequence for a gene to the mRNA that results after transcription and processing. What is the same? Different? Is it possible to get more than one mRNA sequence from a single gene? 1 page limit.
2.A double stranded DNA molecule, shown here, has a gene called Pente that produces a polypeptide that is five (5) amino acids long.
a.Shown is one strand of a DNA molecule. Fill in the complementary strand.
b.Label the 5’ and 3’ ends of each strand to reflect the correct orientation for the Pente gene.
c.What is the sequence of the mRNA and polypeptide that are produced from the Pente gene?
d.What single nucleotide change would reduce the size of the pente protein from five amino acids to three amino acids? What would be a good name for this new allele?
e.Assume that the bottom strand is the template and it is transcribed from left to right. What is the RNA produced from this template DNA? What is the amino acid sequence of the resulting polypeptide?
f.Are there any other possible genes in this short stretch of DNA? If so, what is the amino acid sequence of the resulting peptide?
3’-ATGATTTACTCAGCAATAACAACTATGTAA-5’
3.You have isolated several mutants of the following wild-type protein. Show the point mutation or deletion (using mRNA sequence information) that caused each of the changes. Use this information to determine the sequence of the template DNA for this gene as accurately as possible.
Wild-type protein: Met-phe-tyr-met-arg-gln
Mutant 1: Met-phe-thr
Mutant 2: Met-phe-tyr-met
Mutant 3: Met-phe-tyr-met-thr-gln
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