Biochemistry

Clostridium Sporogenes not growing in nutrient broth

Clostridium Sporogenes not growing in nutrient broth

I'm working with a student who cannot grow Clostridium sporogenes in nutrient broth incubated at 37C (over 6 hrs). Is he missing anything, PH, any key ingredient, etc?
note: Bacteria was freshly acquired

GSan T   51   1 Reply

bio_man

Chair conformation and stability

Chair conformation and stability

A)  Use the provided structures of D-mannose and L-mannose and complete the structures
of these two sugars in chair conformation by drawing the remaining hydroxyl (OH)
groups at the correct positions (axial or equatorial). Predict t

Anonymous   213   2 Replies

Anonymous

Chair Conformations

Chair Conformations

Draw all of the possible chair conformations, identify cis and trans conformations, and arrange the order of stability for these conformations.


A) 1,3-dihydroxycyclohexane



B) 1,4-dibromocyclohexane

Anonymous   147   2 Replies

Anonymous

Locate the H+ and OH- ions from the water molecule

Locate the H+ and OH- ions from the water molecule

locate the H+ and OH- ions from the water molecule that was split (hydrolyzed) in this reaction, showing esculin hydrolysis.

last problem on the attachment #4 not sure about this one..

12

bronze   10624   18 Replies

Alexander Nikanshin

When preparing an acetate buffer at pH 4.5 with 0.01 M solutions of acetic acid (pKa = 4.8) and ...

When preparing an acetate buffer at pH 4.5 with 0.01 M solutions of acetic acid (pKa = 4.8) and ...

When preparing an acetate buffer at pH 4.5 with 0.01 M solutions of acetic acid (pKa = 4.8) and sodium acetate, the volume of acetic acid needed would be ____ the volume of sodium acetate solution.
 
  a. equal to
  b. less than half

mahi   218   3 Replies

Lettyyys

Procedure to identify protein markers for pathology diagnosis

Procedure to identify protein markers for pathology diagnosis

I'm a biochemistry student and I'm stuck on this question:Propose a procedure to identify protein markers for pathology diagnosis ?

Chou Rar   81   1 Reply

Anonymous

Resonance structures

Resonance structures

Provide all possible resonance structures for the following compound.

Anonymous   90   1 Reply

Anonymous

Lewis Base and Acid

Lewis Base and Acid

Using the listed compounds, note what compounds are able to function as a Lewis Base and/or a Lewis Acid.

NH3

CO32-

Cl-

H[

Anonymous   65   1 Reply

Anonymous

Electron-pushing mechanism for acid-base reactions

Electron-pushing mechanism for acid-base reactions

Use the Electron-Pushing mechanism to illustrate and complete the following acid-base reactions. Specify what the acid, base, conjugate acid, conjugate base, and what compound is donating the proton

A)  HCO3-[/s

Anonymous   72   1 Reply

Anonymous

Hybridization

Hybridization

Determine the hybridization of atomic orbital for the carbon and oxygen atoms in the following compound (use the given table and numbering for the specification of atoms). What are the bond angles between C1-C2-C3 and C3-C4-05? What kinds of orbitals

Anonymous   74   1 Reply

Anonymous

Define & contrast these 3 terms...

Define & contrast these 3 terms...

Fermentation,
Oxidative phosphorylation,
Aerobic resperation

Kabbces   9403   1 Reply

bolbol

Name and describe the three ways to add phosphate to ADP to make ATP

Name and describe the three ways to add phosphate to ADP to make ATP

Name & describe 3 ways to add phosphate to ADP to make ATP

Kabbces   791   1 Reply

bolbol

Calculate the pH of a 0.2 M acetate buffer (pKa = 4.77) that contains twice as much acid as ...

Calculate the pH of a 0.2 M acetate buffer (pKa = 4.77) that contains twice as much acid as ...

Calculate the pH of a 0.2 M acetate buffer (pKa = 4.77) that contains twice as much acid as conjugate base.

alpsi   1433   13 Replies

Anonymous

Reaction speed is linearly proportional to substarte concentration over the whole range of substrate ...

Reaction speed is linearly proportional to substarte concentration over the whole range of substrate ...

Reaction speed is linearly proportional to substarte concentration over the whole range of substrate concentrations

A) True
B) False

SkövdeUni   649   1 Reply

bolbol

An ammonia buffer contains NH3:NH4+ in a ratio of 0.4 moles:0.6 moles (pK = 9.75). What will be the ...

An ammonia buffer contains NH3:NH4+ in a ratio of 0.4 moles:0.6 moles (pK = 9.75). What will be the ...

An ammonia buffer contains NH3:NH4+ in a ratio of 0.4 moles:0.6 moles (pK = 9.75). What will be the pH if you add 0.01 moles of HCl to this buffer?
 A) 8.98
  B) 9.04
  C) 9.25
  D) 9.46
  E) 9.52

Angela26kristin   304   3 Replies

jianhong9991

What structural feature is most responsible for chlorophyll's ability to absorb

What structural feature is most responsible for chlorophyll's ability to absorb

What structural feature is most responsible for chlorophyll's ability to absorb light?
A) The magnesium ion.
B) The conjugated double-bonds in the tetrapyrrole ring.
C) The phytol side chain.
D) An aromatic side chain attache

mikael   390   1 Reply

bolbol

The main difference between photosynthesis and respiratory electron transport is

The main difference between photosynthesis and respiratory electron transport is

The main difference between photosynthesis and respiratory electron transport is (are)
A) the tetrapyrole ring of the pigments involved.
B) the coenzymes that carry electrons.
C) the sources of excited electrons.
D) the formation

mikael   393   1 Reply

bolbol

Chlorophyll molecules can exist in ________ different states.

Chlorophyll molecules can exist in ________ different states.

Chlorophyll molecules can exist in ________ different states.
A) 2
B) 3
C) 4
D) 5

mikael   419   1 Reply

bolbol

Photosynthesis by green sulfur bacteria and cyanobacteria are different because

Photosynthesis by green sulfur bacteria and cyanobacteria are different because

Photosynthesis by green sulfur bacteria and cyanobacteria are different because green sulfur bacteria ________.
A) have only cyclic photosynthesis
B) use photosystem I, but not photosystem II
C) use both photosystems I and II
D) u

mikael   310   1 Reply

bolbol

Which use only type I reaction centers for photosynthesis?

Which use only type I reaction centers for photosynthesis?

Which use only type I reaction centers for photosynthesis?
A) Heliobacteria.
B) Purple bacteria.
C) Cyanobacteria.
D) Algae.

mikael   255   1 Reply

bolbol

What family of genes are frequently carried by bacterial transposons

What family of genes are frequently carried by bacterial transposons

What family of genes are frequently carried by bacterial transposons?
A.  Antibiotic resistance
B.  F factor
C.  Origins of replication
D.  Restriction endonucleases
E.  Toxin production

couple007   543   1 Reply

bolbol

Photosynthesis in PS I is non-cyclic because

Photosynthesis in PS I is non-cyclic because

Photosynthesis in PS I is non-cyclic because
A) it is slower than those in PS II.
B) reaction centers must be reduced by electrons from another source.
C) reaction centers are more tightly packed in the photosystem complexes.
D)

mikael   640   1 Reply

bolbol

Reactions to extract electrons from water require ________ to reverse electron f

Reactions to extract electrons from water require ________ to reverse electron f

Reactions to extract electrons from water require ________ to reverse electron flow.
A) powerful oxidizing agents
B) powerful reducing agents
C) dark reactions
D) ATP

mikael   468   1 Reply

bolbol

Photon capture by plant chloroplasts is more efficient than in bacteria because

Photon capture by plant chloroplasts is more efficient than in bacteria because

Photon capture by plant chloroplasts is more efficient than in bacteria because they contain
A) both PS I and PS II.
B) more chlorophyll.
C) LHII, a light harvesting complex.
D) both cyclic and noncyclic photophosphorylation.

mikael   431   1 Reply

bolbol

The enzyme glycoprotein peptidase is irreversibly inhibited by penicillin, which forms a covalent ...

The enzyme glycoprotein peptidase is irreversibly inhibited by penicillin, which forms a covalent ...

The enzyme glycoprotein peptidase is irreversibly inhibited by penicillin, which forms a covalent adduct with a serine residue on the enzyme. From this observation which statement logically follows?
 a. Serine must be protonated for this enzyme

Greenspotsock   359   3 Replies

bio_man

Which of the following reactions IS NOT INVOLVED in regulation of the phosphoryl

Which of the following reactions IS NOT INVOLVED in regulation of the phosphoryl

Which of the following reactions IS NOT INVOLVED in regulation of the phosphorylase kinase cascade?
A.  formation of cyclic AMP.
B.  activation of protein kinase.
C.  phosphorylation of certain amino acids of phosphoryla

couple007   613   1 Reply

bolbol

Wavelengths of Light

Wavelengths of Light

   Dear Biochemistry Community:
     Could it be that the plant pigments in leaves help to filter out which wavelengths of  light are not necessary in plant metabolism?  As an example:  The green of chlorophyll filters out

StanleyJoe   158   2 Replies

Thomas Kistner

Question about bases of DNA

Question about bases of DNA

The question consists of a table in which the percentage of different bases of DNA molecule in a human are written like this:
Source of DNA.     %Adenine.      %Guanine.     %Thymine.     %Cytosine

Happyhuman001   227   3 Replies

Anonymous

Chemicals that bind to DNA by intercalating between

Chemicals that bind to DNA by intercalating between

Chemicals that bind to DNA by intercalating between the bases
A.  can cause frame shift mutations
B.  can cause deletion mutations
C.  can cause insertion mutations
D.  are generally planar hydrophobic molecule

couple007   437   1 Reply

bolbol

DNA As Polymer

DNA As Polymer

Dear Biochemistry Community:
     Could anyone think of the DNA molecule as a polymer, its constituent amino acids as monomers, and the RNA molecules as being organic
catalysts, that is, as enzymes?  Thank you.

StanleyJoe   103   1 Reply

bio_man

Complete the following reaction for the proteolytic cleavage

Complete the following reaction for the proteolytic cleavage

The activation of chymotrypsinogen, a zymogen, requires the cleavage of a bond between Arg-15 and Ile-16, and the subsequent attraction of Ile-16 and Asp-194. Complete parts (a) and (b). a) Complete the following reaction for the proteolytic cleavage

123

locklearl   23402   39 Replies

TacoSauce71

What is the product of the reaction catalyzed by rubisco in the Calvin cycle? A

What is the product of the reaction catalyzed by rubisco in the Calvin cycle? A

What is the product of the reaction catalyzed by rubisco in the Calvin cycle?
A) Dihydroxyacetone phosphate.
B) 3-phosphoglycerate.
C) Ribose 5-phosphate.
D) Fructose 6-phosphate.

mikael   362   1 Reply

bolbol

Attempts to genetically modify rubisco to help increase food production might in

Attempts to genetically modify rubisco to help increase food production might in

Attempts to genetically modify rubisco to help increase food production might include trying to ________.
A) enhance the carboxylation reaction and suppress the oxygenation reaction
B) suppress the carboxylation reaction and enhance the oxy

mikael   297   1 Reply

bolbol

Dialysis Tubing Experiment

Dialysis Tubing Experiment

Dialysis tubing is a cellulose material used to make model cells for experiments in osmosis and diffusion. These model cells do not use living cell membranes. A student made a model cell by pouring distilled water into some dialysis tubing. The stude

Anonymous   121   1 Reply

Anonymous

Xenobiotic metabolism: phase 1 reduction enzyme

Xenobiotic metabolism: phase 1 reduction enzyme

Phase biotransformation reactions can be oxidations, reductions or hydrolysis of the xenobiota molecule.
Phase 1 oxidations are catalyzed by the cytocrome P450 enzyme, abbreviated with the CYP acronym: the active site of this enzyme is a prorphy

Marco Atzori   196   1 Reply

Anonymous

An unknown amount of He gas occupies 30.5 L at 2.00 atm pressure and 300 K. What is the weight of ..

An unknown amount of He gas occupies 30.5 L at 2.00 atm pressure and 300 K. What is the weight of ..

An unknown amount of He gas occupies 30.5 L at 2.00 atm pressure and 300 K. What is the weight of the gas in the container?
 a. 2.48 g c. 9.91 g
  b. 4.95 g d. 19.8 g

Rot wow   166   1 Reply

Anonymous

To express Tat Protein in CD4 T cells without pre-activation

To express Tat Protein in CD4 T cells without pre-activation

Hello All
My experiment plan: To express Tat protein in CD4 T cell without pre-activation. I have tried different vectors and virus such as MND-AAV6 EGFP, CMV-AAV6 EGFP, AdRGD EGFP, SFFV-Lentivirus EGFP, MSCV-Lentivirus EGFP , HIV-VSV-G-Luc and

Harry Tian   160   N/A

Harry Tian

Which term is not used for a photosystem or a part of a photosystem?

Which term is not used for a photosystem or a part of a photosystem?

Which term is not used for a photosystem or a part of a photosystem?
A) PS I.
B) PS II.
C) Special pair.
D) Light complexes.
E) Antenna pigments.

mikael   802   1 Reply

bolbol

table sugar is placed into a negative control flask. Explain the effect this would have

table sugar is placed into a negative control flask. Explain the effect this would have

For this question, im a little confused as to what the effects would be on the other nutrients. For example, I think the sugar might reduce but I still think I'll need an explanation for the answer as I'm confused as to how it would effect

Krystal209   116   5 Replies

Krystal209

Can someone explain induction by IPTG And why DE3 is essential in induction?

Can someone explain induction by IPTG And why DE3 is essential in induction?

Can someone explain induction by IPTG

And why DE3 is essential in induction?

anatomy99   561   1 Reply

bolbol