lab

Pre-Lab Questions Nitrogen fixation is a natural process by which inert or unreactive forms of nitrogen are transformed into usable nitrogen. Why is this process important to life? Given what you have learned about the hydrogen bonding shared between nucleic acids in DNA, which pair is more stable under increasing heat: adenine and thymine, or cytosine and guanine? Explain why. Cytosine and Guanine would be because they have 3 hydrogen bonds and adenine and thymine have 2 Which of the following is not an organic molecule: methane (CH4), fructose (C6H12O6), rosane (C20H36), or ammonia (NH3)? How do you know? Ammonia (NH3) because it does not have carbon Experiment 1: Testing for Proteins Data Tables Table 1: Testing for Proteins Results Sample Initial Color Final Color Is Protein Present? 1. Albumin Solution yellow Dark purple yes 2. Gelatin Solution Dark yellow Dark black/purple yes 3. Glucose clear Dark blue with clear tone no 4. Water clear Light blue with clear tone no 5. Unknown foggy Foggy light blue no Post-Lab Questions Record your hypothesis about what will happen when Biuret solution is mixed with the solutions from test tubes 1, 2, 3, and 4 here. Be sure to use scientific reasoning to support your hypothesis. My hypothesis is that by mixing the Blue color of the biuret reagent with the yellow color in test tube one will create a green color in test tube one, mixing with the yellow color in test tube two it will create a green color, mixing the biuret with the clear color in test tube three and four it will create a blue color and then mixing the biuret with the foggy in test tube 5 will create a light foggy blue color. Write a statement to explain the molecular composition of the unknown solution based on the results obtained during testing with the Biuret solution and each sample solution. Based on both test tube one and 2 you can assume the protein is present as they were the ones that turned purple and the rest did not. Diet and nutrition are closely linked to the study of biomolecules. How should you monitor your food intake to ensure the cells in your body have the materials necessary to function? As a way to monitor you food intake you can familiarize with the six food groups. Also recognize the essential vitamins and minerals your body needs. Then you can link what your body needs with the types of food you can get them in. There are other types of reagents used to determine what type of biomolecule a substance is. For example, copper ions present in Benedict’s reagent reacts with the free end of any reducing sugars, such as glucose, when heated. Originally blue in color, these copper ions are reduced by the sugar, and produce an orange-red colored precipitate. Alternatively, iodine-potassium iodide (IKI) may also be used when working with starch. IKI contains special tri-iodine ions which interact with the coiled structure of a starch polymer. Prior to a reaction, the IKI displays a yellow-brown color; however, after reacting with starch, a dark purple or black color is presented. The molecule pictured below produced a blue color when tested with Benedict’s reagent, a yellow color when tested with IKI, and a violet color when tested with Biuret reagent. Based on the structure shown below and these chemical results, what kind of biomolecule is this? Protein