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SlideshowReport

Consolidated Hfr map of E. coli

Description
(a) The 100-minute genetic map of E. coli. Genes of bacterial operons (discussed in Section 12.2) are boxed. The origin of replication (oriC) is seen at 84 minutes. (b) A 2.5-minute segment (minutes 42.5–45) of the E. coli time-of-entry map in comparison with a segment of approximately 500,000 base pairs of the E. coli genome derived from E. coli genomic sequencing. Selected genes between 42.5 minutes and 45 minutes on the time-of-entry map (upper) are aligned with their positions in the genome sequence map (lower) to illustrate the compatibility of the two mapping approaches(a) The 100-minute genetic map of E. coli. Genes of bacterial operons (discussed in Section 12.2) are boxed. The origin of replication (oriC) is seen at 84 minutes. (b) A 2.5-minute segment (minutes 42.5–45) of the E. coli time-of-entry map in comparison with a segment of approximately 500,000 base pairs of the E. coli genome derived from E. coli genomic sequencing. Selected genes between 42.5 minutes and 45 minutes on the time-of-entry map (upper) are aligned with their positions in the genome sequence map (lower) to illustrate the compatibility of the two mapping approaches

(a) Time-of-entry map Bacteria operons N l—I [BCGFEK\|amBM/ DEBA [m Replication leu [ origin C7 A>mbl ’ g—xyl ' T P ma/ Q argR ‘ argG ara “~ ,<’//fl,,wl,mmfi \\\\\\ x” [H.fif/UEAEEQRQ ‘‘‘‘‘‘ (b) Compaging ségments of Hfr time-of-entry map and sequenced genome ‘‘‘‘‘‘‘‘‘ g m A. B. D “ ~~~~~~~ ACDFGHIJKLMNOPQR U _ _, _, £ Q (ABCDE) (FGH) ‘~\ i 7 E<>=3<EESEE $1 I‘el Siam m Confounded Face E e figéfiwmsmé mfi Eafi gets‘sa a Time in 43 44 45 minutes ) K \ fliL rcsA asnT ‘3an aan sch hisL hisB vsr [ll] gnaw cobU sme alum“ gnd rfc rbega/F cpsG Genes “333511 ii P [1] Eh‘I-D Eli. [IIEEIHQ hi IlllIli-Iilm -»-.._...4 _._...>> Base pa i rs 1,800,000 2,000,000 2,200,000
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