Transcription control of PHO5 in Saccharomyces cerevisiae

(a) Transcription is repressed in high-phosphate conditions. (b) In low-phosphate conditions, Pho4 joins Pho2 at UASp1, and NuA4 directs acetylation of nearby nucleosomes. The SWI/SNF complex attaches, leading to the ejection of nucleosomes −1 to −4. RNA polymerase II and general transcription factors initiate PHO5 transcription(a) Transcription is repressed in high-phosphate conditions. (b) In low-phosphate conditions, Pho4 joins Pho2 at UASp1, and NuA4 directs acetylation of nearby nucleosomes. The SWI/SNF complex attaches, leading to the ejection of nucleosomes −1 to −4. RNA polymerase II and general transcription factors initiate PHO5 transcription

(a) High phosphate results in transcription repression é PHOS UASp1 UASp2 TATA Nucleosome —5 —4 —3 —2 —1 +1 number (b) Low phosphate results in transcription activation UASp1 UASp2 TATA -5 —4 -3 —2 —1 +1 Nucleosome ejection, SWVSNF binding, and transcription activation SWI/SNF /. _ . UASp1 UASp -5 TATA +1