The generation of Golden Rice

(3) Synthesis of beta-carotene ilPSY 2 — 4 icanso Lycopene Lycopene 3 lchv 5 laLCY. 3ch 4 [ B—HYD 6 la-HYD. B-HYD B-carotene (b) Recombinant plasmids th regulatory sequence Daffodil PSY Bacterial CRT/ Gt1 regulatory Selectable marker sequence Right T-DNA GRI plasmid Left T—DNA rFirstgenerlairon golden rice (GRl): Daffodil phyzoeeeflsymhase gene ‘ (PSY) and bacterial C RTI gene from Erwin/a uredovora are driven ‘ With rice gluIeIIn-l (Gil) endosperm regulatory sequences (green). Bacterial CRTI Maize PSY m GRII plasmid Selectable marker Left T—DNA Right TrDNA Second-generation golden rice (GRH): A maize PSY gene was exchanged for me daffodil PSY gene, boosiing me production of ‘ u—carolene. (c) Appearance of wild-type and transgenic rice B-carotene < ‘63 produced in endosperm