P element–mediated transformation in Drosophila

The P element used as a vector contains the gene of interest and 1 also the rosy+ gene conferring wild-type eye color but lacks a functional transposase. A second plasmid supplies the transposase l activity in trans. l Vector plasmid Second plasmid Gene of interest P element 3’ transcriptional transposase \ terminator P element I inverted repeat end sequences Co-inject plasmids Amp“ and ori are for into rosy embryos propagation of plasmids in bacteria. J Transposase activity inserts the P element. l . ts * Female Male ntegration even occur rosy‘ rosy‘ in some cells giving rise to gametes, which leads I to some progeny with x the rosy' gene and the gene Of interest. Mate injected adult females with rosy '/rosy ' males. rosy rosy rosy rosy rosy- Hflflflfl Progeny with red eyes must have inherited a complementing rosy‘ transgene and also the gene of interest.