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How gene drive alleles can spread through populations

Description
(a) (b) (t) Mendelian inheritance Gene drive inheritance Heterozygous fl . Heterozygous . mutant 3 { ’l W“ type gene drive allele :% 7’“ W'Id type a A at -_ at \ , flack L ATJN RT 2-». RT 7% NT a» mfg at] a» Wait 957 R» l l l ‘ l l l ' l ’ l E ' i l l at on e" o- 7% o as %%%%%%-% Progeny have a 50% chanre of inheriting the allele if one of their parents is heterozygous. Regions of homology to genome flanking the guideRNA target site 0 0 Cas9 GuideRNA Homologous 3— allele V — -— a Genomic DNA __________V l \ Target site 0 Homologous recombination Homologous —._...,.,_._.,..l recombination “— J 0 _— 9 __..__. __..,_ _____. —___,_ — -— Gene drive allele Homologous gene drive alleles 0 < Cargo CasB GuideRNA > i l i . F
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