Cytoplasmic Male Sterility in Flowering Plants

9 CMS Rf/_ OWild-type N_/_ 9 CMS rf/rf d' Inbred A x Inbred B 9 rf/rf Normal Single-cross progeny - l l "' l a: a m a: c: a: Male sterility Fertility restored / In the absence of the l I In the presence of an Rf PPR protein encoded allele, the PPR protein by the Rf allele, the 3 CMS ORF is transcribed 3 and translated, resulting in an aberrant j gene product and ‘ \ male sterility. rf/rf CMS (male sterile) CMS (male sterile) Lgx (AxB) [ processes the chimeric RNA containing the CMS ORF in such a way that a functional gene product is produced and fertility is restored. d Inbred C x Inbred D 9 Rf/Rf Normal rf/rf CMS (male sterile) Single-cross progeny Rf/rf CMS (male fertile) A (CxD) Double-cross hybrid seed (planted by farmer) $Rf/rf CMS (male fertile) %rf/rf CMS (male sterile)