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SlideshowReport

Gene-transfer processes in bacteria

Description
(a) Conjugation. A single DNA strand transferred during DNA replication in the donor is used to replicate a second strand in the recipient. Subsequent crossing over recombines DNA to form the exconjugant cell. (b) Transformation. A single strand of donor DNA taken across the membrane of the recipient cell recombines with recipient DNA to form the transformant cell. (c) Transduction. A donor DNA fragment encapsulated in a transducing phage is injected into the recipient cell, where it recombines to form the transductant cell(a) Conjugation. A single DNA strand transferred during DNA replication in the donor is used to replicate a second strand in the recipient. Subsequent crossing over recombines DNA to form the exconjugant cell. (b) Transformation. A single strand of donor DNA taken across the membrane of the recipient cell recombines with recipient DNA to form the transformant cell. (c) Transduction. A donor DNA fragment encapsulated in a transducing phage is injected into the recipient cell, where it recombines to form the transductant cell

(a) Conjugation Conjugation DNA pilus replication Donor Recipient Bacterial DNA-strand transfer Crossover in Exconjugant cell cell chromosome from donor to recipient recipient cell cell produced by recombination (b) Transformation 6 A I -- “ —» © — (>5 — ’1 . § Recipient chromosome DNA fragments Recipient cell Crossover of Transformant from donor takes up donor donated strand cell bacteria DNA with recipient chromosome (c) Transduction _ _ Transducmg Bacterial X chromosome ‘ Donor Bacterial Recipient cell chromosome cell 0 Bacteriophage e Phage DNA 9 Transducing o Transducing a Donor DNA @ Transductant cell attaches to 3 injection phages package phage attaches injected and produced by donor cell. leads to donor DNA to a recipient recombined recombination. donor instead of cell. with the chromosome phage DNA. recipient fragmentation. chromosome.
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