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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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jordant.22 jordant.22
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6 years ago
Describe how DNA is transferred from a donor F-positive to a recipient F-negative cell.
 
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6 years ago
Ans: The TraY protein binds to an origin of transfer site, oriT, on the F plasmid. This causes the protein TraI to bind. TraI is a relaxase that nicks the DNA, attaches to the 5 end, and unwinds the nicked strand from its complement. This strand passes to the recipient cell, leaving a single circular strand in the donor cell. Both strands are duplexed by DNA replication, and the recipient cell's DNA recircularizes to result in one complete F plasmid in each of the two cells.
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