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Home Q & A Board Biology-Related Homework Help Biochemistry
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element41skater element41skater
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13 years ago
A graduate student wants to do in vitro translation of a prokaryotic message and adds purified prokaryote small and large ribosomal subunits, f-met-tRNAf-met and all 20 charged tRNAs, a template mRNA with a known start codon and GTP to a flask in an appropriate buffer.  After a 6 hour incubation no new proteins were found. Whats missing?
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#1
13 years ago
Could it be a release factor? A release factor is a protein that allows for the termination of translation by recognizing the termination codon or stop codon in a mRNA sequence.

It could also be elongation factors that are missing. Elongation factors are a set of proteins that facilitate the events of translational elongation, the steps in protein synthesis from the formation of the first peptide bond to the formation of the last one.
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