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4pedrozas 4pedrozas
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8 years ago
Many of the 2500 students and employees of a public school system developed diarrheal illness on May 2, 2007. the cafeteria served chicken on that day. On May 1, part of the chicken was placed in water-filled pans and cooked in an oven for 2 hours at a dial setting of 177 degrees Celsius . The oven was turned of and the chicken was left overnight in the warm oven. The remainder of the chick was cooked for 2 hours for a steam cooker and then left in the device overnight at the lowest possible setting, 43 degrees Celsius. Two serotypes of a gram-negative, cytochrome oxidase negative, lactose-negative rods were isolated from 32 patients.
what is the pathogen? How could this outbreak have been prevented? List test that could be done to identify this time of pathogen in the laboratory? What microbiological test were done to test for this organism? What is mean by cytochrome oxidase negative and lactose negative?
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Educator
8 years ago
what is the pathogen?

This illness is caused by Salmonella bacteria.

How could this outbreak have been prevented?

  • Wash your hands
  • Keep your food preparation areas clean
  • Avoid unpasteurized foods
  • Cook and store your food at the appropriate temperatures
  • Be careful when handling animals
List test that could be done to identify this time of pathogen in the laboratory?

Most subspecies of Salmonella produce hydrogen sulfide, which can readily be detected by growing them on media containing ferrous sulfate, such as is used in the triple sugar iron test. Most isolates exist in two phases: a motile phase I and a nonmotile phase II. Cultures that are nonmotile upon primary culture may be switched to the motile phase using a Craigie tube or ditch plate.

Salmonella can also be detected and subtyped using multiplex or real-time polymerase chain reactions (PCR) from extracted salmonella DNA.

Quote
What is mean by cytochrome oxidase negative and lactose negative?

Salmonella are classified as anaerobic, gram-negative, facultative, rod-shape, non-fermenter, oxidase negative (lack cytochrome oxidase), indole test negative, Methyl red test positive, and H2S are produced from thiosulfate, etc.
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