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Home Q & A Board Biology-Related Homework Help Cell Biology
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juliasarands juliasarands
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12 years ago
I am currently in Microbiology and am having trouble understanding the Operon Model of Gene Expression. Does repression and induction have anything to do with the Operon Model?
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#1
12 years ago
whoo boy, what a can of worms.

 The Operon Model, as you know, is quite an extensive theory.  Yes, repressor proteins do come into play.  The main characters in the model include RNA, mRNA, regulator proteins, structural genes and regulator genes.  To try to explain it here would take a lot of typing, and truthfully it's one of those things that I understand, but unfortunately can't break down very well.  Here's a website I think offers a very clear breakdown of the model.  I hope you find it useful Slight Smile  

http://www.mun.ca/biochem/courses/3107/Topics/Operon.html

good luck in micro. Slight Smile   It's usually a neat class, esp. if you have a good prof.  If you're majoring in micro, watch out for bacteriology.  I still have nightmares about that class, LOL
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tommyo0729tommyo0729
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12 years ago
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#3
12 years ago
Eg.

Lac Operon
      1.  Physical Description - segment of DNA found in some prokaryotes allowing for regulation over protein synthesis (translation)

      2.  Five Components
           a.  Promoter - specific segment of DNA to which RNA Polymerase attaches to begin transcription of mRNA from an operon
           b.  Operator - sequence of nucleotides located between the promoter and structural genes
           c.  Regulator - sequence of DNA found anywhere on the bacterial chromosome; codes for the production of repressor protein
           d.  Repressor - protein which can bind to the operator, thus inhibiting the promoter from binding, which, in turn, leads to NO transcription because RNA Polymerase was not able to bind or move along the DNA
           e.  Inducer - substance which can activate or inactive a repressor
                Example in Lac Operon:
                1)  Lactose is the inducer
                2)  If lactose is present, it binds to and inactivates the repressor by causing it to fall off the operator
                3)  If lactose operator is free of repressor, then RNA Polymerase is able to bind.
                4)  If RNA Polymerase can bind to the lac operon, then B-galactosidase is produced, and lactose is catabolized into glucose and galactose for energy
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