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Biostudent510 Biostudent510
wrote...
Posts: 19
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10 years ago
Hi guys, sorry, but I have absolutely no idea how to even approach this problem, or where to even start. I don't even know what you call this table!

Complete the table. Several auxotrophic point mutants in Neurospora are treated with various agents to see if reversion will take place. The following results were obtained (a plus sign indicates reversion; HA causes only G • C → A • T transitions).

Mutan   5-BU   HA   Proflavin   Spontaneous reversion   a) The nature of the original mutation event   b) Name a possible mutagen (hint: this should be a group name, not a specific name such as 5-BU)
Sample   +    –    –    +   transition   Base analogs

1

   –    –    –    –      
2


    –    –    +    +      
3



    –    –    –    +      
4


   +    +    –   +      

a. For each of the five mutants, describe the nature of the original mutation event (hint: your answer should not be the reversion) at the molecular level.

b. For each of the five mutants, name a possible mutagen that could have caused the original mutation event. (Spontaneous mutation is not an acceptable answer.)

ehhhh sorry about the table formatting getting all mixed up :/

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Replies
wrote...
10 years ago
Answer:

a. and b.

Mutant 1: most likely a deletion. It could be caused by radiation.

Mutant 2: because proflavin causes either additions or deletions of bases and because spontaneous mutation can result in additions or deletions, the most probable cause was a frameshift mutation by an intercalating agent.

Mutant 3: 5-BU causes transitions, which means that the original mutation was most likely a transition. Because HA causes GC-to-AT transitions and HA cannot revert it, the original must have been a GC-to-AT transition. It could have been caused by base analogs.

Mutant 4: the chemical agents cause transitions or frameshift mutations. Because there is spontaneous reversion only, the original mutation must have been a transversion. X-irradiation or oxidizing agents could have caused the original mutation.

*****THIS WAS THERE TOO BUT I DON'T SEE A 5th MUTATION ON YOUR PROBLEM

Mutant 5: HA causes transitions from GC-to-AT, as does 5-BU. The original mutation was most likely an AT-to-GC transition, which could be caused by base analogs.
Source  http://www8.umoncton.ca/umcm-filion_martin/Solutions/iga_10e_sm_chapter_16.pdf
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