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cb2015 cb2015
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6 years ago
When photosynthetic pigments are extracted from the chloroplasts of green plants, they have a higher
  tendency to fluoresce than pigments within intact chloroplasts. Explain this phenomenon. What will be an ideal response?

Question 2

Propose an appropriate solvent to be used in the extraction of photosynthetic pigments from
  chloroplasts. Explain your choice. What will be an ideal response?
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wrote...
6 years ago
Answer to q. 1

Concepts to Consider: Extracted pigments are removed from the thylakoid membranes where other
components of the electron transport chain are located. This causes the uncoupling of the pigment
molecules from electron acceptors, which results in a greater likelihood of fluorescence than in
coupled pigment-acceptor systems

Answer to q. 2

Concepts to Consider: A suitable solvent would be any hydrophobic organic solvent such as acetone
or petroleum ether. The reason for this is that the pigments are hydrophobic and are embedded in a
hydrophobic thylakoid membrane
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