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Home Q & A Board Biology-Related Homework Help Cell Biology
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sma1394 sma1394
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12 years ago
Theoretically, there should be no uptake, but when I did my results, the bacteria had indeed taken up the plasmid DNA even when I didn't do any heat shock?

Does anyone have any explanations for this?
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wrote...
#1
12 years ago
Maybe you added chemical reagents that help make the membrane permeable? I think calciumchloride can do that.

EDIT: Or it might be that you already have bacteria with the altered plasmid. They can exchange their plasmids which then is viewed by you as 'uptake'.
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#2
12 years ago
Transformation - uptake of plasmids, is commonly induced by calcium chloride.
wrote...
#3
12 years ago
Yes they can, but much less efficiently.  Sharing plasmids between different species or even different genera has been documented in nature.
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