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penguinlover penguinlover
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11 years ago
I'm having a hard time understanding DNA replication so hopefully you guys can clear some stuff up for me.

1) Does the DNA move through the helicase or does the helicase move across the DNA when it gets unwound?

2) Are there two Primases since there is a lagging strand and leading strand? Both strand need a primer (the leading only needs one) so I'd think there are two Primases but then are they both attached to the helicase?
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11 years ago
1) helicase hydrolyses ATP and moves across the DNA. DNA is a huge molecule, while helicase is comparedly tiny.

2) Primosome is a protein comblex responsible for creating the RNA strands during replication (at procaryotes). Primosome contains two active enzymes that creates the RNA strands (primers). Of course, these enzymes are not identical (their function is slightly different lagging and leading strands). The primosome somehow interacts with helicase, but I'm not sure exactly how.
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