Your task is to design an experiment to test prepared laboratory samples to identify.
1) a normal person
2) an insulin dependent patient
3) a mild diabetes mellitus insulin-independent patient
You are given three beakers containing 100 mL of prepared samples marked patient A, patient B, and patient C. The random samples represent a normal person, an insulin-dependent patient, and an insulin independent patient.
Equipment Available - All normal laboratory equipment such as test tubes, test tube racks, beakers, drop plates, graduated cylinders, thermometers, measuring spoons, hot plates, test tube tongs, oven mitts, masking tape, towels, and marking pencils.
Reagents and preparations available - Biurets reagent, Benedict's solution, Urine analysis glucose test strips, amylase, pepsin, 2% HCl solution, sodium bicarbonate, bile extract, soap, and distilled water.
QUESTIONS
1) Based on the information provided, form a hypothesis for your experiment. Please include the words glucose, higher or lower, normal, and diabetes mellitus.
2) Using the lab materials available, create a procedure to identify which sample could belong to each of the three patients. Keep in mind that you must perform TWO laboratory tests.
HINT
( The procedure must contain numbered sentences. Your procedure must be clear so that any Biology 30 student can take your directions, use the above experiment, and obtain expected results)
HINT
(Color changes with the indicator are not instant (time) )
HINT
(How many test tubes will you use? Three is not the correct answer)
3) Identify the independent variable in this experiment
4) Identify the dependent variable in this experiment
5) Identify the controlled variable in this experiment
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