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lynch1234 lynch1234
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6 years ago
In the Sanger, or chain termination, method of DNA sequencing, incorporation of the nucleotides stops because
 
  A) the dideoxynucleotide analogs have no 3-OH group to continue DNA synthesis.
  B) the dideoxynucleotide analogs have no 5-OH group to continue DNA synthesis.
  C) the dideoxynucleotide analogs have no 2-OH group to continue DNA synthesis.
  D) the mixture is heated to 92C to stop DNA synthesis at specific time intervals.
  E) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
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wrote...
6 years ago
Answer: A
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