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Which HSC quantitation method would be optimal for verification of late-stage engraftment?

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Which HSC quantitation method would be optimal for verification of late-stage engraftment?

Which HSC quantitation method would be optimal for verification of late-stage engraftment?

a. Manual count of MNCs
b. CD34 enumeration by flow cytometry
c. Cell culture for CFUs
d. Bone marrow exam for stem cells

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