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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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princay princay
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10 years ago
A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 2 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 20 plaques. What is the initial density of bacteriophages in the original 1 mL?
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wrote...
#1
10 years ago
0.1mL in 9.9mL is 100 times diluted...thus 5 times dilution means 100^5 is dilution factor....thus 12 plaques x 100^5=12x10^10 bacteriophages present in original 1 mL
wrote...
#2
10 years ago
correction: 12x10^10 in 0.1 mL therefore in initial 1mL there are 12x10^10 x 10=12x10^11 bacteriophages/mL
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