I will be presenting a seminar about this article.. potential questions?

Hey ehd123, please download it and add it as an attachment. It won't let us access...

Hello bioman
I'm so sorry I didn't notice that
Here's the pdf

How can combining lineage conversion and further lineage specification help to circumvent long-standing obstacles for the reprogramming of human cells into hematopoietic cells?

What clinical potential does do these approaches have?

Please specify examples pertaining to in vivo or in vitro approaches.

Hey Padre, thank you so much these questions are great. Are these right?

How can combining lineage conversion and further lineage specification help to circumvent long-standing obstacles for the reprogramming of human cells into hematopoietic cells?

Well, some of the obstacles are that the producing, characterizing and differentiation  the cells is a length process, and may lead to neoplastic and malignant formations as well as fail to engraft. The lineage conversion and specification technique enable engraftment and maturation in an in vivo niche safely and without cancerous formations in a much shorter period of time.
I think :/

What clinical potential does do these approaches have?
It aids in bone marrow transplantations and stem-cell based therapies as well as blood transfusions
I think also :/


Please specify examples pertaining to in vivo or in vitro approaches.
I don't get this question 

That about their methods here, the methods the scientists used.

*I meant talk about*

Oh I see.
Well, an example of the in vivo method would be the injection of human-converted fibroblasts CD34+/CD45- cells into the femurs of mice only to end up with human CD45+/CD45- murine cells and their detection in distant parts of the hematopoietic system such as the contralateral bone marrow, spleen and femur (injection site). Thus, showing that the in vivo niche was responsible for the maturation and migration of the cells.

In vitro would be culturing the cells on a dish to test their phagocytic capacity. For example, when the researchers transduced fibroblasts with Sox2 and miR-125b  obtaining CD34+ cells that were further differentiated into monocyte-like cells CD14+. The phagocytic capacity was tested in vitro by incubating some of these cells with opsonized beads with human serum. Another example would be the second differentiation test using CFU assays to confirm that maturation took place in vivo by being able to form a colony in a dish

Right?

I've been through a panel like this before, ehd123, and most questions are derived from the discussion section, so make sure you understand what conclusions they have made and how they derived those conclusions.

In terms of answering your question whether you're right, I don't know. I didn't read the whole article - too time consuming - but I did skim through it enough to find some reasonable questions.

That's a great tip! Will do! I understand the conclusions, but I am just a little afraid I would be asked about the details of the experiments. For example, how many cells were transnduced here, percentage of surviving cells is taken as the percentage from the initial or from the proliferated total number of cells, which cells were generated, these results were compared or normalized to what cells, etc. It is these tiny details I really don't like.

Also, must I include the discussion parts in my presentation? Or just be very familiar with it in case I was asked something and had to refer to it?

This seminar is not  my thesis, however the professor asking and judging will be treating it as such. He really refers to dodging all sorts of difficult questions as "grilling" the presenter, and this Friday, it's my turn to be grilled 

Yes, include the discussion parts. Or, you can include the results and take bits and pieces from the discussion and include that on each results slide.


It's good practice then.You'll know what to improve on from there.

Thank you for the help Will do my best