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7 years ago
DNA sequencing using the dideoxy sequencing method involves PCR reactions using Taq polymerase, which we learned in this chapter can introduce errors because it lacks a proofreading mechanism.
 
  What precaution(s) can be taken in genomic or other sequencing projects to minimize the possibility of producing DNA sequences with errors?
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7 years ago
The most common precaution taken is sequencing a fragment of DNA in both directions. Any replication errors will show up as mismatches, which could be resolved by resequencing that fragment of DNA.
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