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Twatson22 Twatson22
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7 years ago
List the steps involved in constructing a knockout mutant in yeast.
 
  What will be an ideal response?
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7 years ago
Most commonly, a deletion module is constructed with a selectable marker (such as an antibiotic resistance gene). Transformants are selected using the antibiotic resistance gene. The module recombines with the target knockout gene, and successful replacements can be confirmed with PCR.
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