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Darkflower73 Darkflower73
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Posts: 669
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6 years ago
The medium or media of choice used to recover Legionella is:
 
  a. buffered charcoal yeast extract (BCYE).
  b. Regan-Lowe agar.
  c. Lwenstein-Jensen medium.
  d. blood and chocolate agars.



A pharyngeal specimen is inoculated on cystine-tellurite blood agar, modified Tinsdale agar, and MacConkey agar. After 48 hours of incubation, black colonies with a brown halo are observed on the Tinsdale agar and gray colonies are isolated from the cystine-tellurite agar. However, no growth is observed on the MacConkey agar. Which one of the following statements explains this discrepancy?
 
  a. The MacConkey agar is most likely ex-pired.
  b. The MacConkey agar should be incubated an additional 24 hours to observe growth.
  c. The pH of the media may be altered, causing an environment that is too acidic for the organism to grow.
  d. No discrepancy is present; this organism does not grow on MacConkey agar.



The biochemical description of Myroides is:
 
  a. oxidase positive, motile, and rapid urease positive.
  b. urease positive and has a fruity odor.
  c. resistant to vancomycin and will grow on MacConkey agar at 42C.
  d. nonmotile and both urea positive and phenylalanine deaminase positive.



The following species, P. aeruginosa, P. fluorescens, Pseudomonas putida, P. veronii, Pseudomonas monteilii, and Pseudomonas mosselii, all produce a water-soluble, yellow-green pigment called:
 
  a. pyocyanin.
  b. pyomelanin.
  c. pyorubrum.
  d. pyoverdin.



The natural habitat of Chryseobacterium sp. can best be described as the:
 
  a. mucous membranes of animals.
  b. gastrointestinal tract of humans and other animals.
  c. natural environment.
  d. genitourinary tract of animals.
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Replies
wrote...
6 years ago
A
Specimens for culture should be inoculated to two agar plates for the recovery of Legionella, at least one of which is BCYE without inhibitory agents. This medium contains charcoal to detoxi-fy the medium, remove carbon dioxide (CO2), and modify the surface tension to allow the or-ganisms to proliferate more easily. BCYE is also prepared with N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) buffer and the growth supplements cyste-ine (required by Legionella), yeast extract, <-ketoglutarate, and iron. A second medium, BCYE base with polymyxin B, anisomycin (to inhibit fungi), and cefamandole, is recommended for specimens such as sputum that are likely to be contaminated with other flora.

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D
The organism is C. diphtheria and therefore can grow on Tinsdale agar but fails to grow on MacConkey agar.

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B
Urea hydrolysis is a key test for Myroides spp., which is also distinguished by production of a characteristic fruity odor.

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D
P. aeruginosa, P. fluorescens, P. putida, P. veronii, P. monteilii, and P. mosselii comprise the group known as the fluorescent pseudomonads. These species all produce pyoverdin, a wa-ter-soluble, yellow-green or yellow-brown pigment that fluoresces under UV illumination. P. aeruginosa is the only species that produces pyocyanin.

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C
As environmental inhabitants, these organisms may be found in various niches. Most notable in terms of clinical relevance is their ability to survive in hospital environments, especially in moist areas. Although they are not considered part of normal human microbiota, these species can co-lonize a patient's respiratory tract during hospitalization, probably as a result of exposure to a contaminated water source or medical devices.
Darkflower73 Author
wrote...
6 years ago
Easily the best answer, TY and have a wonderful day
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