Coactivators are:
A. necessary only for initiation of transcription of housekeeping genes.
B. factors that bind to other proteins, not to DNA directly.
C. factors that complex directly with RNA Pol II to form a preinitiation complex.
D. factors that bind to enhancers.
Which of the following statements regarding transcription only apply to prokaryotes and not to eukaryotes?
A. A region of the promoter about 30 nucleotides upstream of +1 contains AT-rich base pairs.
B. The RNA start site is designated +1.
C. A region of the promoter about 10 nucleotides upstream of +1 contains AT-rich base pairs.
D. Important sequence elements are frequently found downstream of +1.
E. All of the choices given apply to eukaryotes.
The first topoisomerase identified was DNA topoisomerase I of bacteria. How was the activity assayed?
What will be an ideal response?
Basal transcription factors are:
A. necessary only for initiation of transcription of housekeeping genes.
B. factors that bind to other proteins, not to DNA directly.
C. required at every Pol II promoter.
D. factors that bind to enhancers.
When sedimented by ultracentrifugation, polyoma virus DNA separates into three bands:
form I (20S), form II (16S), and form III (14S). What do these forms represent, and what property of DNA did discovery of these three forms lead to?
What will be an ideal response?
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