In a typical sequencing reaction, the amount of dNTPs is much greater than of the ddNTPs. Predict the results if equal amounts of both types of nucleotides were used in each reaction. After gel electrophoresis and visualization:
A. mostly long fragments would be present and no sequence could be read close to the primer.
B. mostly short fragments would be present and only sequences very close to the primer could be read.
C. the sequence could be read normally; the dNTP/ddNTP ratio does not affect the products of the reaction.
D. only the purine reactions would show termination and be read from the gel.
E. no bands would be visible because the high concentration of ddNTPs would inhibit the activity of the polymerase.
Which of the following best describes the tRNA binding sites of the ribosome?
A. Only one of the three sites can be occupied by a tRNA molecule at any given time.
B. Each tRNA that associates with the ribosome must first bind in the P site and then move to the A site.
C. The P site contains the tRNA molecule covalently bound to the growing chain of amino acids.
D. All tRNAs leave the ribosome through the A site.
Why is mutation of DNA repair genes the most harmful to the cell?
CPEs are:
A. cytoplasmic sequences for polyadenylation.
B. nuclear polyadenylation sequences.
C. cytoplasmic proteins that disrupt the interaction between eIF4E and eIF4G.
D. None of the above is correct.
How can the disruption of epigenetic markers result in cancer?
What will be an ideal response?
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